Relationship Between Sperm Head Vacuoles and Sperm DNA Alterations in Infertile Men

Launched by UNIVERSITY HOSPITAL, ROUEN · Dec 4, 2013

Keywords

ClinConnect Summary

The use of intracytoplasmic sperm injection (ICSI) has greatly improved the treatment of severe male infertility, especially for men with oligo-astheno-teratozoospermia (OAT). This in vitro fertilization procedure allows the direct injection of a single spermatozoon into an oocyte. The sperm used for ICSI is selected under a microscope at a 400x magnification. Several studies have reported that de novo chromosomal abnormalities are increased in children born from ICSI , thereby raising the question of the genetic quality of spermatozoa used for ICSI.

A method called MSOME (high magnificati...

Gender

MALE

Eligibility criteria

• Inclusion Criteria:
• • men
• • 18 years old or more
• • affiliated to social security
• * with isolated teratozoospermia or oligo-astheno-teratozoospermia according to the WHO guidelines (2010):
• • sperm concentration \< 15 million per ml or sperm number \< 39 million per ejaculate
• • progressive motility \< 35%
• • morphologically normal forms \< 50% according to the modified David's classification (Auger et al., 2001
• • mobile spermatozoa selected by density gradient centrifugation \> 1 million, in previous spermograms
• • with normal constitutional karyotypes (46, XY)
• • signed an informed consent
• Exclusion Criteria:
• • with abnormal constitutional karyotypes
• • with a major alteration of sperm parameters, with mobile spermatozoa selected by density gradient centrifugation \< 1 million
• • under tutorship or guardianship by court order