Evaluation of Next-Generation Sequencing-based Metabarcoding Versus Culturing for Microbiological Assessment in Infectious Keratitis

Launched by AARHUS UNIVERSITY HOSPITAL · Dec 21, 2021

Keywords

ClinConnect Summary

This clinical trial is looking at two different methods for identifying the germs that cause infectious keratitis, a serious eye condition that can threaten your sight. Traditionally, doctors use a technique called culturing, where samples are taken and grown in a lab to find out what kind of bacteria or other organisms are present. However, a newer method called Next Generation Sequencing is now available, which can quickly detect the DNA of these microorganisms without needing to grow them in a lab. This study will compare these two approaches to see which is more effective in identifying the cause of keratitis.

To participate in this trial, you need to be between the ages of 18 and 74 and have a specific type of eye issue known as corneal infiltrate, which is a cloudy area on the cornea (the clear front part of the eye) that is at least 2 mm wide, or 1 mm if it is located in the central part of the cornea. If you join the study, you can expect to undergo both testing methods to help researchers understand which one works better for diagnosing this condition. This information could lead to improved treatment options for future patients with infectious keratitis.

Gender

ALL

Eligibility criteria

• Inclusion Criteria:
• • - Patients with a corneal infiltrate of minimum 2 mm in diameter or of 1 mm diameter within the central 6 mm of the cornea
• Exclusion Criteria:
• • - Patients below 18 years of age