Search / Trial NCT06228742

Molecular Mechanisms Underlying Anabolic Resistance to Protein Intake During Muscle Disuse

Launched by UNITED STATES ARMY RESEARCH INSTITUTE OF ENVIRONMENTAL MEDICINE · Jan 18, 2024

Trial Information

Current as of October 07, 2024

Recruiting

Keywords

Description

Warfighters that sustain musculoskeletal injuries often experience decreased muscle loading and activation post-injury (i.e., muscle disuse) that results in a rapid loss of muscle mass and function. Loss of muscle under these conditions is attributed to a persistent negative net muscle protein balance (muscle protein synthesis \[MPS\] \< muscle protein breakdown \[MPB\]) that results, in part, from a blunting of MPS in the postprandial state. Nutritional interventions that optimize postprandial MPS have been suggested as countermeasures for this "anabolic resistance" that develops during di...

Gender

ALL

Eligibility criteria

  • Inclusion Criteria:
  • * Men and women aged 18-39 years.
  • * Body mass index (BMI) between 18.5-30 kg/m2
  • * Healthy without evidence of chronic illness (e.g., diabetes, cardiovascular disease, Crohn's disease) or musculoskeletal injury as determined by the USARIEM Office of Medical Oversight (OMSO) or home duty station medical support.
  • * Routinely participate in aerobic and/or resistance exercise at least 2 days per week.
  • * Willing to refrain from alcohol, smoking, smokeless nicotine products (includes e-cigarettes, vaping, chewing tobacco), caffeine, and dietary supplements (i.e., vitamin D, probiotics) during the run-in diet, immobilization phase, and final testing day of the study.
  • * Supervisor approval for federal civilian employees and non-human research volunteer (HRV) active-duty military personnel stationed at Natick Soldier System Center (NSSC).
  • * Biological females must have normal menstrual cycles between 26-32 days in duration; 5 menstrual cycles within the past 6 months; or on continuous hormonal contraception (i.e., intrauteraine device (IUD) or oral contraceptives without placebo).
  • Exclusion Criteria:
  • * Musculoskeletal injuries that may interfere with the safe use of crutches.
  • * Personal or family history of thrombosis, or prior diagnosis of deep vein thrombosis (DVT) or pulmonary embolism (PE).
  • * Metabolic or cardiovascular abnormalities, gastrointestinal disorders, neuromuscular disorders, lower-limb amputation, or muscle/bone wasting disorders (e.g., diabetes, cardiovascular disease, Crohn's disease, etc.).
  • * Significantly abnormal blood clotting as determined by OMSO or home duty station medical support.
  • * Allergy to lidocaine (or similar local anesthetic)
  • * Present condition of alcoholism, anabolic steroid use, or other substance abuse issues as determined by OMSO or home duty station medical support.
  • * Blood donation within 8-wk of beginning the study.
  • * Pregnant, trying to become pregnant, and/or breastfeeding (results of urine pregnancy test and self-report for breastfeeding will be obtained before body composition testing).
  • * Unwilling or unable to consume study diets or foods provided due to personal preference and/or food allergies.
  • * Unwilling or unable to adhere to study physical restrictions (i.e., no structured physical activity or recreational activity beyond activities of daily living) 24 hours before and during immobilization, and the final testing day.
  • * Unwilling or unable to keep the knee brace on and walk with crutches during the immobilization phase.

About United States Army Research Institute Of Environmental Medicine

The United States Army Research Institute of Environmental Medicine (USARIEM) is a premier military research organization dedicated to enhancing the health and performance of soldiers through innovative scientific investigation. With a focus on environmental physiology, nutrition, and injury prevention, USARIEM conducts rigorous clinical trials and studies aimed at optimizing soldier readiness and resilience in diverse operational conditions. By integrating advanced research methodologies and interdisciplinary collaboration, USARIEM plays a crucial role in informing military policies and practices, ultimately contributing to the well-being and effectiveness of military personnel.

Locations

Natick, Massachusetts, United States

People applied

Timeline

First submit

Trial launched

Trial updated

Estimated completion

Not reported

Discussion 0