Detection of Mycobacterium Tuberculosis in Blood of TB Patients and Their Contacts in Uganda

Launched by QUEEN MARY UNIVERSITY OF LONDON · Dec 27, 2024

Keywords

ClinConnect Summary

For many years, development of such a test was not considered realistic due to the paucibacillary nature of LTBI and uncertainty regarding the cellular location of Mtb bacilli in LTBI. However, the investigators and others have recently reported that Mtb DNA can be detected in the peripheral blood of latently infected TB contacts in both high- and low-burden settings. In our studies, which utilized digital polymerase chain reaction (dPCR) to detect Mtb DNA in peripheral blood mononuclear cell (PBMC) subsets of TB contacts in Ethiopia, signal was detected more frequently in extracts of CD34+...

Gender

ALL

Eligibility criteria

• TB patients (Group A):
• Inclusion Criteria:
• • Able and willing to give written informed consent to participate
• • Age ≥18 years
• • New diagnosis of smear-positive or Xpert-confirmed pulmonary TB
• • At least one asymptomatic adult household contact likely to fulfil TB contact eligibility criteria below
• Exclusion criteria:
• • More than 7 days of anti-TB treatment taken
• • Confirmed or suspected pregnancy (female participants)
• • Existing medical condition which in the opinion of the investigator may adversely affect the participant's safety or ability to participate in the study.
• Recent TB contacts (Group B):
• Inclusion Criteria:
• • Able and willing to give written informed consent to participate
• • Age ≥18 years
• • Household contact with an index case of smear-positive pulmonary TB in Group A above.
• Exclusion Criteria:
• • Clinical or radiological features of active TB
• • Current or previous anti-TB treatment
• • Confirmed or suspected pregnancy (female participants)
• • Existing medical condition which in the opinion of the investigator may adversely affect the participant's safety or ability to participate in the study.
• Historical TB contacts (Group C):
• Inclusion Criteria:
• • Able and willing to give written informed consent to participate
• • Age ≥18 years
• • Participant in previous TB household contact study
• Exclusion Criteria:
• • Clinical or radiological features of active TB
• • Current or previous anti-TB treatment
• • Confirmed or suspected pregnancy (female participants)
• • Existing medical condition which in the opinion of the investigator may adversely affect the participant's safety or ability to participate in the study.