Comparison of IL-8, FGF-1 and VEGF Levels in Pulpal Tissue, GCF and Dentinal Fluid Samples of Healthy, Reversible and Irreversible Pulpitis Diagnosed Teeth

Launched by KIRIKKALE UNIVERSITY · Apr 12, 2025

Keywords

ClinConnect Summary

This clinical trial is studying different levels of specific proteins (called biomarkers) in the pulp tissue of teeth to understand how they relate to different types of pulp conditions: healthy pulp, reversible pulpitis (mild inflammation), and irreversible pulpitis (more severe inflammation). Researchers will collect samples from 50 patients' teeth to measure levels of IL-8, FGF-1, and VEGF, which may help in diagnosing and treating these dental conditions better.

To participate in this study, you might be eligible if you have healthy teeth, or if you have mild or severe pulpitis without other dental issues. Participants can expect a fairly straightforward process: samples will be collected from their teeth using gentle, non-invasive methods, followed by routine dental treatment as needed. It's important to note that patients who have certain health conditions, like diabetes or gum disease, or who have recently taken antibiotics, may not be able to join the study. Overall, this trial aims to enhance our understanding of dental pulp health, which could lead to improved treatments for patients experiencing tooth pain.

Gender

ALL

Eligibility criteria

• Inclusion Criteria:
• Control group:
• • Unrestored, caries-free vital teeth with healthy pulp
• • Orthodontically indicated teeth for extraction
• • wisdom teeth indicated for extraction in terms of oral hygiene
• • teeth that require root canal treatment for prosthetic purposes
• Reversible pulpitis group:
• • Teeth that react mildly to cold, hot, sour or sweet stimuli
• • Teeth with no history of spontaneous pain
• • Teeth that respond to cold test and electric pulp test within normal limits
• • Teeth that are not sensitive to chewing or percussion
• Irreversible pulpitis group:
• • Teeth with a history of continuous moderate or severe spontaneous pain
• • Teeth sensitive to percussion
• • Teeth without periapical pathology
• Exclusion Criteria:
• • Patients requiring antibiotic prophylaxis.
• • Patients with diabetes or haematological diseases.
• • Patients who have used antibiotics or anti-inflammatory drugs in the last three weeks.
• • Patients who are pregnant during treatment.
• • Patients with intense plaque and tartar on their teeth, redness and bleeding in the gums.
• • Patients with severe gingivitis, generalised periodontitis or periodontal pocket depth of more than 3 mm.
• • Patients with no response to pulp sensitivity tests, presence of sinus tract, presence of periapical pathology on radiography.
• • Teeth without pulp exposure after caries removal