Comparison of Aquaporin -4, -5, -9 and IL-8 Levels in GCF, Dentin Fluid and Pulp Samples

Launched by KIRIKKALE UNIVERSITY · Apr 20, 2025

Keywords

ClinConnect Summary

This clinical trial is studying the levels of specific proteins (called Aquaporins and IL-8) in different types of dental tissue and fluids. The researchers want to see how these levels change in people with healthy teeth compared to those with a painful condition known as irreversible pulpitis, which is a serious inflammation of the tooth pulp. The goal is to understand if there is a connection between these protein levels and the state of the teeth.

To participate in this study, you must be between 18 and 64 years old and scheduled for routine root canal treatment at the Kırıkkale University Dental School. You should not have any known health issues or be pregnant. Participants will provide samples of dental tissue and fluids before their treatment begins, and these will be tested in a lab. This research could help improve our understanding of dental health and how to better treat conditions like pulpitis in the future.

Gender

ALL

Eligibility criteria

• Inclusion Criteria:
• • Patients aged 18 to 64 years.
• • Patients without any known systemic disease.
• • Patients requiring root canal treatment for prosthetic purposes (Healthy pulp group).
• • Patients with third molars (wisdom teeth) indicated for extraction due to orthodontic or other clinical reasons (Healthy pulp group).
• • Patients diagnosed with symptomatic irreversible pulpitis based on the criteria established by the American Association of Endodontists (AAE) (Symptomatic irreversible pulpitis group).
• • Patients scheduled for routine root canal treatment at the Department of Endodontics, Faculty of Dentistry, Kırıkkale University.
• • Patients who have provided written informed consent to participate in the study.
• Exclusion Criteria:
• • Patients with any known systemic disease.
• • Pregnant women or those suspected of being pregnant.
• • Patients who have used antibiotics, anti-inflammatory drugs, or antidepressants within the past 4 weeks.
• • Patients with significant dental plaque or calculus, gingival redness or bleeding, severe gingivitis, generalized periodontitis, or periodontal pockets deeper than 4 mm.
• • Patients with teeth showing internal or external root resorption.
• • Patients for whom complete rubber dam isolation cannot be achieved.
• • Patients with teeth exhibiting signs of necrosis, apical lesions, swelling, or presence of a sinus tract.
• • Patients with immature teeth presenting open apices.
• • Patients with third molars (wisdom teeth) that are not fully erupted or have been previously diagnosed with pericoronitis.