MicroRNA Profile As a Biomarker of Liver Damage in Different Types of Liver Donors.

Launched by MAR DALMAU · Sep 23, 2024

Keywords

ClinConnect Summary

Between October 2019 and May 2021, perfusates, liver biopsies and serum samples were collected prospectively from adult deceased organ donors in Vall d\'Hebron University Hospital, Barcelona, Spain.

Samples from adult human brain death donors (n=10) and donors after circulatory death (n=10) were analyzed for the presence of miR-122, miR-148, miR-155, miR-22, miR-222 and ratio miR122/miR222, target biomarkers of liver injury selected from the literature.

To set the reference miRNA profiles, a subgroup of donors with unusable grafts due to significant steatosis (n=3) were analyzed.

Don...

Gender

ALL

Eligibility criteria

• I) Main donor groups (DBD and DCD):
• Inclusion Criteria:
• • Adult deceased liver donors
• Exclusion Criteria:
• • Pediatric donors (less than18 year-old)
• • Donors for re-transplantation cases
• • Donors who underwent split or partition liver technique to obtain two small grafts for two recipients (adult and pediatric).
• II) Discarded DBD grafts:
• Inclusion Criteria:
• • - Unusable grafts for transplantation due to moderate (30% to 60%) or severe (more than 60%) steatosis in liver biopsies.
• Exclusion Criteria:
• • Unusable grafts discarded for other reasons.
• • Pediatric donors (less than18 year-old)
• • Donors for re-transplantation cases
• • Donors who underwent split or partition liver technique to obtain two small grafts for two recipients (adult and pediatric).
• III) Control group:
• Inclusion criteria:
• • - Same inclusion criteria as the living liver donor program in our center: 18 to 60 years old, normal liver function, ultrasound without signs of liver disease and steatosis less than 30%.
• Exclusion criteria:
• • - Patients with preoperative deranged liver function tests.